Monitoring FOXP3+ Tregs Cells Sensitivity To Low Doses of IL-2 using p-STAT-5 detection in Ex-Vivo Whole Blood Samples

Monitoring FOXP3+ Tregs Cells Sensitivity To Low Doses of IL-2 using p-STAT-5 detection in Ex-Vivo Whole Blood Samples

14 Jul 2015

The detection of phospho-epitopes by flow cytometry is a long and inefficient process, required multiple washing steps and dedicated buffers and protocols which can be harmful to certain epitopes, like the FOXP3 marker. Interleukin-2 (IL-2) is a key player in the immune system, affecting lymphocytes and the regulatory system. This poster demonstrates a fast and reliable method for gentle sample preparation allowing monitoring of regulatory T cell (Treg) sensitivity to IL-2.

Kaluza Analysis Software

Beckman Coulter Diagnostics

Kaluza is a revolutionary flow cytometry analysis software solution designed for high content data. Kaluza provides visualization tools, speed and an innovative simplicity to the flow community. With features designed to make your life easier in the lab, Kaluza aims to provide a greater understanding of the data generated by modern cytometers. Kaluza employs technology that allows for real time analysis of high content files allowing you to spend your valuable time on discovery. Features: Layout flexibility Multiple report layouts per sample Analyze directly from report or plot sheet Interactive plots on reports

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Gallios™ Flow Cytometer

Beckman Coulter Diagnostics

The Gallios Flow Cytometer is a research system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection. Designed to fit your needs, today and tomorrow, the Gallios flow cytometer provides efficient acquisition of excellent quality data from up to 10 colors with advanced optical design for enhanced sensitivity for multicolor assays. With customized forward scatter, to achieve the most accurate size measurement, and optimized electronics for high resolution and fast, accurate processing, the Gallios was designed for your research needs.The Gallios offers up to four highly reliable, solid-state lasers in standard red and blue, with violet and yellow lasers available as an option. By incorporating the optional 405nm violet laser and an optional 561nm yellow laser, the Gallios Flow Cytometer offers a greater choice of fluorochromes to perform sophisticated multicolor experiments. With the new 561nm laser system you can analyze multiple fluorescent proteins simultaneously, as well as benefit from a greater ability to detect red fluorescent proteins. Easily interchangable optical filters facilitate detection of a variety of dyes and wavelengths. Gallios™ Flow Cytometer Features: Available in 4 configurations Innovative laser cartridges Plug and play filters allow rapid instrument reconfiguration between experiments Efficient delivery of light Electronics design incorporates fast, accurate measurement of signals over a wide range of event rates

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :RegulatoryPharmaceutical regulations impact on all areas of drug development, manufacture and control and supply. Services are provided from the early stages of drug development and clinical trials through to dossier submission, approval and marketing. Regulatory also includes submission of analytical studies from the pharmaceutical product and safety. ImmunologyImmunology is the branch of medical science that covers the study of all aspects of the immune system of multicellular organisms.Biomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.EpitopeLymphocytes
Monitoring FOXP3+ Tregs Cells Sensitivity To Low Doses of IL-2 using p-STAT-5 detection in Ex-Vivo Whole Blood Samples