Minimizing Phospholipid Interference During LC-MS/MS Analysis of Vitamin D Metabolites in Serum for Clinical Research
28 Nov 2018Interferences by matrix components can be challenging when measuring serum levels of vitamin D metabolites by LC-MS/MS, particularly lysophosphatidylcholines. They are challenging to remove during sample preparation and difficult to separate chromatographically. This white paper describes an approach for method optimization using the Oasis PRiME HLB solid phase extraction plate, from Waters.
Related Products
Request Quote for All Products
Oasis® µElution Plate
WatersThe Oasis® uElution plate format combined with the Oasis chemistries allow elution volumes as low as 25uL, eliminating the time consuming evaporation step, producing very clean extracts with a 5 to 10 fold increase in concentration.The Oasis® family of sample extraction products is designed to simplify and improve your sample preparation by combining the right sorbent chemistry, device format and methodology. Achieve robust, selective, and sensitive solid-phase extraction [SPE] methods without worrying about low recoveries caused by breakthrough, sorbent drying, pH limitations, and undesirable silanol activity.Oasis® SPE sorbents—covered by eight U.S. patents—are unique in their purity, reproducibility, stability, and retention characteristics. Today, they are the most widely used polymeric SPE products in bioanalytical laboratories.
Xevo TQ-S micro Triple Quadrupole Mass Spectrometer
WatersHigh Performance from a compact design with rapid, reliable and reproducible quantitative data.