ResourceSeparations
Method Development for Rapid SFC Analysis of Vitamin D3 and Related Compounds
11 Jan 2019By using the advantages of Supercritical Fluid Chromatography (SFC), namely high permeability and diffusibility, generally higher resolution can be achieved by SFC analysis with shorter run times than by HPLC. Base-line separation of Vitamin D3 (cholecalciferol), three related compounds (pre-cholecalciferol, 5,6-trans-cholecalciferol, tachysterol3 ) and the antioxidant (α-tocopherol) is achieved using an Alcyon SFC Triart Diol column under SFC conditions with shorter analysis time, which is one quarter the time for the normal phase HPLC method.
Links
Tags
UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Chromatography Method DevelopmentChromatography method development is the process of designing and optimizing chromatographic techniques to separate and analyze complex mixtures. This includes selecting appropriate stationary and mobile phases, determining flow rates, and optimizing detection methods. Successful method development is essential for high-quality results in fields like pharmaceuticals, food safety, and environmental analysis. Explore chromatography method development tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Vitamin D