Metacomplex Formation & Binding Affinity of Multivalent Binding Partners
24 Jun 2017This application note describes the use of (CG-MALS) to investigate formation of a metacomplex between streptavidin (SA), and a bivalent antibody (Ab) in solution. A Calypso® II connected to an inline UV/Vis concentration detector and DAWN® HELEOS® MALS detector were used to create automated composition gradients. This article highlights the uniquie suitability of CG-MALS for investigating complex systems of macromolecules in solution.
Related Products
Request Quote for All Products
Calypso™ Biomolecular Interaction Analysis System
Waters | Wyatt TechnologyAbsolute characterization of protein-protein and other macromolecular interactions with composition-gradient multi-angle light scattering.
DAWN™
Waters | Wyatt TechnologyThe DAWN is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution, offering the highest sensitivity and widest range of molecular weight, size, concentration and optional features.