Media Exchanges Using Unattached 3D Spheroid Models

3D cell culture methods encourage cell-cell and cell-matrix interactions and also promote more physiological cell morphologies and behaviors compared to cells grown on flat 2D surfaces. Due to the need to generate the most in-vivo like data during in vitro drug discovery of toxicology testing, 3D spheroids are often used in long-term experimental procedures, and in microplate format to increase throughput. Since these procedures may extend to weeks, media exchange and dosing steps are critically important. When incorporating unattached spheroids, care must be taken not to evacuate or damage the spheroid suspended in the culture media.