Measurement of TOC and TN in Marine Water Algae Culture Solution

23 Jul 2015

The culturing of microalgae in various types of non-edible biomass has been receiving considerable attention in recent years. Various nutrients, such as nitrogen compounds, are added to these types of microalgae cultures but these must be managed within an appropriate range. This application note describes the use of Shimadzu’s TOC-L Series Combustion Type Total Organic Carbon Analyzer in the direct measurement of the total organic carbon (TOC) and total nitrogen (TN) content in high-salt samples such as seawater, without requiring dilution of the samples.

TOC-L Total Organic Carbon Analyzer

Shimadzu Corporation

  Consisting of four models, including PC-controlled and standalone versions, the TOC-L uses Shimadzu’s 680° C combustion catalytic oxidation method to efficiently analyze all organic compounds. It features automatic sample acidification and sparging, as well as an automatic dilution function that reduces sample salinity, acidity and alkalinity. This significantly extends the use of catalysts and combustion tubes. The TOC-L Series features variable syringe sizes when the sample volume is limited. High-precision mass flow controllers ensure uninterrupted carrier gas for accurate analysis. A zero-maintenance Peltier cooler used for maximum water vapor removal ensures accurate analysis. In addition, an on-board air purifier may eliminate the need for high-purity air during testing. The optional salt kits permit 12 times more salt to be analyzed before maintenance is required. The ASI-L autosampler can use three different vial sizes for various applications, while the smaller OCT-L autosampler can use any vial size for up to 8 or 16 samples. TOC-L models can accept particulates up to 500 microns or 800 microns with the optional particulate kit. For larger particles, solids, soils and sludge, the SSM-5000 is the ideal solution. The TNM-L can be added for simultaneous TOC and Total Nitrogen analyses without increasing the size of the footprint.  

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Measurement of TOC and TN in Marine Water Algae Culture Solution