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Managing Sample Volume for Micro Bioreactor Analytics

TAP Biosystems
16 Sept 2014

Many traditional analyzers are not designed for use with micro scale bioreactors, so managing the culture volume when running ambr15 experiments requires some planning. This technical note presents several methods that users currently employ to acquire necessary off-line data when running the ambr15 system. These include the use of low volume analyzers, reducing sample frequency, prioritization of assays, dilution of samples, and sampling a single vessel among replicates.

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ambr15™ Bioreactor

TAP Biosystems

ambr15™ mimics the characteristics of classical bioreactors at microscale (10-15ml), by using cost effective, disposable micro bioreactors, controlled by an automated workstation. ambr15 enables the rapid evaluation of multiple bioreactor cultures at microscale, increasing productivity in cell line development with significant savings on materials and labour. ambr15 is widely used by major Pharma and biologics companies, academic and research institutes as a reliable microscale model for a range of upstream processes.Features & Benefits: A better tool for cell line selection and development - closely mimics the conditions of larger scale bioreactors Simplified adoption of Quality by Design (QbD) principles for cell culture development Significant reduction in cost per experiment More experiments with less operator time for set up and clean down Applications: Cell line selection Process optimisation Media development Media feed strategies QbD and DoE studies Transient Transfection

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.

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Managing Sample Volume for Micro Bioreactor Analytics