LPS Activation of MAPK Signaling in Whole Blood Samples

6 Oct 2014

LPS (lipopolysaccharide) is known to activate multiple signaling pathways in peripheral blood monocytes via the Toll-Like Receptor 4 (TLR4) complex. Following LPS exposure, monocytes rapidly activate all three major MAP Kinase pathways – ERK, p38, and SAP/JNK, in addition to PI3 Kinase and IKK/NFkB pathways. This application note describes a procedure for monitoring these signaling pathways in whole blood samples using flow cytometry.

Gallios™ Flow Cytometer

Beckman Coulter Diagnostics

The Gallios Flow Cytometer is a research system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection. Designed to fit your needs, today and tomorrow, the Gallios flow cytometer provides efficient acquisition of excellent quality data from up to 10 colors with advanced optical design for enhanced sensitivity for multicolor assays. With customized forward scatter, to achieve the most accurate size measurement, and optimized electronics for high resolution and fast, accurate processing, the Gallios was designed for your research needs.The Gallios offers up to four highly reliable, solid-state lasers in standard red and blue, with violet and yellow lasers available as an option. By incorporating the optional 405nm violet laser and an optional 561nm yellow laser, the Gallios Flow Cytometer offers a greater choice of fluorochromes to perform sophisticated multicolor experiments. With the new 561nm laser system you can analyze multiple fluorescent proteins simultaneously, as well as benefit from a greater ability to detect red fluorescent proteins. Easily interchangable optical filters facilitate detection of a variety of dyes and wavelengths. Gallios™ Flow Cytometer Features: Available in 4 configurations Innovative laser cartridges Plug and play filters allow rapid instrument reconfiguration between experiments Efficient delivery of light Electronics design incorporates fast, accurate measurement of signals over a wide range of event rates

(4)

Links

Tags

LPS Activation of MAPK Signaling in Whole Blood Samples