Long-Term Live-Cell Imaging of Angiogenesis in Co-Culture

This poster demonstrates the use of IncuCyte, a compact new fluorescent imaging instrument, in the quantification of multiple aspects of tube formation in living co-cultures of HUVEC and normal human fibroblasts as part of models to show resistance to anti-angiogenic drugs. Neo-angiogenesis in adults is central to several diseases including cancer, arthritis, diabetes, macular degeneration and wound healing. Angiogenesis inhibitors targeting vascular growth factors, signalling pathways and matrix proteases are demonstrably effective in both preclinical models and clinical use, but the effectiveness of this form of therapy can be transitory due to evasive and intrinsic resistance. IncuCyte will facilitate the discovery of new classes of anti-angiogenic drugs, as well as aid in understanding the mechanism of action of existing drugs.