Label Free Phenotype MicroArray Analysis of Cellular Energetics and Apoptotic Activity using Microplate Reading and Phase Contrast Imaging

This poster demonstrates a method to combine cellular metabolic Phenotype MicroArrays™ and automated, digital widefield phase contrast microscopy to screen differential nutritional pathway usage of test cells, in addition to potential pathway interruption by an apoptotic compound. Maintenance of a 5% CO2-95% air atmosphere at 37 ºC in the Cytation™ 5 plate chamber ensured sample integrity and enabled measurements of both cell morphology by image analysis and tetrazolium dye reduction rates, a reporter of cell energy production and cell viability in different nutritional environments.