ResourceLife Sciences
Label Free Phenotype MicroArray Analysis of Cellular Energetics and Apoptotic Activity using Microplate Reading and Phase Contrast Imaging
6 May 2015This poster demonstrates a method to combine cellular metabolic Phenotype MicroArrays™ and automated, digital widefield phase contrast microscopy to screen differential nutritional pathway usage of test cells, in addition to potential pathway interruption by an apoptotic compound. Maintenance of a 5% CO2-95% air atmosphere at 37 ºC in the Cytation™ 5 plate chamber ensured sample integrity and enabled measurements of both cell morphology by image analysis and tetrazolium dye reduction rates, a reporter of cell energy production and cell viability in different nutritional environments.
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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microarray AnalysisMicroarrays, also known as biochips, are used for the detection and analysis of multiple genes, proteins, antibodies, or biomarkers on a single microchip. This can reveal information on protein or gene expression, single nucleotide polymorphism (SNP), copy number variation (CNV), epigenetics and patient health in clinical diagnostic tests. Discover a range of microarray scanners and prefabricated antibody, protein, RNA and DNA microarrays for your analysis or consider creating your own custom microarrays with a microarray printer. Find the best microarray products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Metabolic Phenotyping