Isolation of Stably Transfected Fluorescent Cells with the S3™ Cell Sorter

Many fields of research, from immunology to neurobiology, require the isolation of cells based on extracellular or intracellular phenotypes. Until recently, cell sorters were expensive, and typically required an experienced operator in a core lab setting. The S3 and S3e™ Cell Sorters, however, can be run by any researcher due to key automated features. Sorts can be carried out reliably and with high purity. This application note shows that stably transfected GFP⁺ HEK 293 and RFP⁺ HEK 293 cells can be sorted to greater than 98% purity from a mixed population using the S3 Cell Sorter.