Isolation and expansion of primary airway epithelial cells

The burden of chronic respiratory diseases is increasing, with chronic obstructive pulmonary disease (COPD), asthma, and cystic fibrosis
affecting millions worldwide. Understanding the molecular mechanisms underlying respiratory diseases requires the use of lab models that recapitulate human airway biology. This application note outlines a full protocol for the isolation and expansion of patient-derived airway epithelial cells using PromoCell technologies.