Interaction Between Immobilized Peptides and Protein from Sera on Cystamine / Glutaraldehyde Functionalized Biochip

SPRi technology (Surface Plasmon Resonance imaging) from Horiba is able to measure multiplexed label-free biolo¬gical interactions in a complex media such as serum. This application note demonstrates the ability to monitor specific interactions between immobilized peptides and proteins issued from non purified immune sera, using a cystamine/glutaraldehyde SAM surface chemistry on the bio¬chip surface. This ability has been illustrated by monitoring the interaction between an ovalbumin peptide fragment and an ovalbumin immunized serum.