IntelliQube® Real-Time Quantitative PCR Performance Characterization
24 Aug 2015The IntelliQube from Douglas Scientific is a fully integrated laboratory instrument that combines liquid handling with real-time quantitative PCR (qPCR) analysis in miniaturized reaction volumes. This application note reports on the performance of the IntelliQube. The performance characteristics of this innovative instrument were determined by a series of PCR-based experiments that interrogated PCR uniformity, resolution, and dynamic range. Additional experiments were performed to demonstrate the utilization of all five fluorescence channels, as well as a multiplex reaction with FAM and HEX reporters.
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IntelliQube™ integrated PCR workflow
LGC Biosearch TechnologiesFully Automated PCR Setup, Amplification and Analysis System. The IntelliQube is the first fully automated and high-throughput instrument featuring seamless integration of liquid handling, thermal cycling, detection, and data analysis. The IntelliQube supports quantitative real-time PCR, end-point PCR, and isothermal chemistries, allowing you to realize the benefits of inline automation enabled by the innovative Array Tape™ consumable. IntelliQube leverages best-in-class multi-functional dispensing for high speed liquid handling in 384- or 768-well Array Tape™. Precise sample dispensing into Array Tape is handled by an interchangeable CyBi® FeliX 96- or 384-channel pipetting head. The IntelliQube supports inline amplification with a Peltier thermal cycling block designed specifically for Array Tape™. Due to a unique block design and surface geometry, both 384- and 768-well Array Tape are supported, without requiring a block change. The IntelliQube features an advanced optical system optimized for inline fluorescence detection in Array Tape. Filtered LEDs are optimized for a variety of commonly used fluorophores excited within the range of 480-620 nm. A high-resolution CCD camera supports detection of fluorescence intensity within 5 detection channels for dyes emitting within the range of 510-705 nm. Multiplex data capture for all 5 optical channels can occur in as little as 10 seconds. Benefits: Walk-away operation Increased efficiency of lab personnel Reduced chemistry costs Increased throughput Reduced consumable costs