ResourceSpectroscopy

Instrumentation for Conventional Raman Microscopy and Total Internal Reflection Raman Microscopy

9 Oct 2014

This application note describes configurations for conventional Raman microscopy and total internal reflection (TIR) Raman microscopy using the Andor HoloSpec ƒ/1.8i spectrograph and Newton 940 CCD.

HoloSpec F/1.8 Imaging Spectrograph

Oxford Instruments Andor

  The new Andor Holospec is the ideal solution for collecting more light and achieving better signal-to-noise ratio faster, which is critical for applications such as micro-Raman mapping, microfluidics, real-time medical diagnosis (point-of-care analyzers) or stand-off bacteriologic agents or explosives detection.   The superb light collection efficiency capabilities of the superfast F/1.8 Andor Holospec spectrograph platform provides a perfect match to Andor’s ultra-sensitive CCD, EMCCD and ICCDs detectors, offering the most sensitive and versatile detection solution on the market for Visible or Near-Infrared spectroscopy. The Andor Holospec is the ideal solution for collecting more light and achieving better signal-to-noise ratio faster, which is critical for applications such as micro-Raman mapping, microfluidics, real-time medical diagnosis (point-of-care analyzers) or stand-off bacteriologic agents or explosives detection. The Holospec also offers aberration-corrected optics for excellent multi-track capabilities, enabling high density fibre optics simultaneous acquisitions with extremely low crosstalk, even on narrow spectroscopy sensors. Its rugged and compact design makes it an ideal tool for challenging industrial or in-the-field applications, while still offering research-grade performance suitable for academic research.

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Newton CCD Camera

Oxford Instruments Andor

The Newton CCD camera is ideally suited to high-performance spectroscopic applications. The high-resolution sensors deliver up to 95% QE. Combined with Andor Technology’s low noise electronics, it provides convenient multi-Megahertz readout and USB connectivity. The camera utilizes a 1024 x 255 array of 26μm² pixels with thermoelectric cooling down to -100°C resulting in negligible dark current. The system offers the best performance for a wide range of spectroscopic applications across VUV-NIR wavelengths.

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Instrumentation for Conventional Raman Microscopy and Total Internal Reflection Raman Microscopy