Ingenio® Electroporation Solution - The High Efficiency, Broad Spectrum Solution for Nucleic Acid Delivery into Primary Cells and Hard to Transfect Cell-lines

29 Jan 2015

The physical technique of electroporation has emerged as a method of choice for nucleic acid delivery into these hard to transfect cells, such as primary cells. Ingenio® Electroporation Solution is a broad spectrum reagent capable of supporting electroporation of plasmid DNA and siRNA into multiple mammalian cell lines. This application note presents a simple protocol for the efficient transfection of cells using Ingenio Electroporation Solution. The method can be further optimized for either exponential decay or square wave pulse types offered on standard electroporators.

Ingenio® Electroporation Solution

Mirus Bio LLC

High efficiency electroporation using any instrumentIngenio® Electroporation Kits and Solution provide a universal, high efficiency, low toxicity solution for electroporation of DNA or siRNA into hard to transfect cell types. Electroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity.Ingenio® Electroporation Solution Features:•High efficiency electroporation of hard to transfect cell lines and primary cells•Compatible with most conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®•Ideal for plasmid or siRNA electroporation

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Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.TransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.
Ingenio® Electroporation Solution - The High Efficiency, Broad Spectrum Solution for Nucleic Acid Delivery into Primary Cells and Hard to Transfect Cell-lines