Improvement in Speed and Reproducibility of Protein Digestion and Peptide Quantitation, Utilizing Novel Sample Preparation Technology in a Full Solution Workflow
Improvement in Speed and Reproducibility of Protein Digestion and Peptide Quantitation, Utilizing Novel Sample Preparation Technology in a Full Solution Workflow
22 Oct 2015Traditional trypsin digest protocols are time consuming, with some protocols taking up to one and a half days to complete and involving multiple steps including evaporation and reconstitution. This adds potential for a high degree of variability. This application note describes a rapid and precise protocol for peptide analysis utilizing immobilized, heat stable trypsin and micro-elution solid-phase extraction (SPE) coupled with next generation ultra-high pressure liquid chromatography and high resolution mass spectrometry (UHPLC-HRMS).
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Thermo Scientific™ SMART Digest™ Kits
Thermo Fisher ScientificObtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits. A significant advance in sample preparation for biopharmaceutical and protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation. Features: Easy and simple Significantly increased speed of sample processing. 3-step sample preparation. Easily automate entire protocol for high-throughput processing. Reliable results Increased reproducibility over existing protocols. Higher sensitivity. Higher data confidence.