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Immunohistochemistry: A guide to investigating cancer hallmarks

SelectScience
11 Jul 2021

The variety of immune cells and interactions present in cancerous tumors makes studying them complex, but also offers plenty of opportunities for immunotherapeutic intervention. Immunohistochemistry (IHC) has not only shown promise in identifying these altered molecular pathways, but also in assessing which of these targets are likely to respond to therapy.

In this eBook, we provide a comprehensive guide to the use of IHC for cancer research and immunotherapy development, speaking to experts in the field about the importance of robustly validated antibodies and how to ensure confidence in your results. Plus, we dive deep into the key players in the immune system and their interactions in the tumor microenvironment (TME).

Download this free eBook to explore:

  • The role of the immune cells and interactions in the TME
  • How to optimize and apply IHC in immuno-oncology
  • The importance of robust antibody validation

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.ImmunohistochemistryImmunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples by using antibodies that bind to target antigens. IHC is widely applied in pathology, immunology, hematology and cancer diagnostics. Explore IHC tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.ImmunotherapyImmunotherapy is a treatment approach that utilizes the body�s immune system to fight diseases, especially cancer. It includes therapies such as monoclonal antibodies, checkpoint inhibitors, and CAR-T cell therapy. Immunotherapy is a rapidly growing area of research in oncology and autoimmune diseases. Browse our peer-reviewed product directory to find the best immunotherapy tools, compare products, check reviews, and get pricing directly from manufacturers.Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.

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Immunohistochemistry: A guide to investigating cancer hallmarks