Identification of 18 Immune Cell Subsets in Human Blood Using a 13-Color Panel

Flow cytometry has become the method of choice for immunophenotyping and identifying specific cellular subsets. Within seconds, it provides a thorough overview of the major cell types that constitute a sample. Using multiple markers simultaneously increases the number of parameters that can be analyzed per run and decreases the amount of starting material required to perform an assay. This can be critical for precious sample material and long-term immunemonitoring studies. In this application note, we demonstrate 13-color immunophenotyping of human peripheral blood mononuclear cells (PBMCs) using the MACSQuant^® Analyzer 16, a compact and reliable benchtop flow cytometer equipped with three lasers.