ResourceDrug Discovery & Development
High sensitivity purity analysis of peptide – oligonucleotide conjugates
23 Feb 2026Oligonucleotide‑based therapeutics are advancing rapidly, but efficient intracellular delivery remains a major challenge due to the negative charge of both oligonucleotides and the cell membrane. Conjugation with membrane‑permeable peptides, such as oligoarginine, improves uptake and strengthens therapeutic potential.
Explore an optimized ion‑pair reversed‑phase (IPRP) method for high‑sensitivity purity analysis of peptide–oligonucleotide conjugates (POCs). Using a model POC (R9‑ER(dec)‑R), the workflow demonstrates reliable differentiation of closely related impurities, supporting accurate quality control and improved confidence in conjugation efficiency for next‑generation oligonucleotide therapeutics.
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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.