High sensitivity purity analysis of peptide – oligonucleotide conjugates

Oligonucleotide‑based therapeutics are advancing rapidly, but efficient intracellular delivery remains a major challenge due to the negative charge of both oligonucleotides and the cell membrane. Conjugation with membrane‑permeable peptides, such as oligoarginine, improves uptake and strengthens therapeutic potential.

Explore an optimized ion‑pair reversed‑phase (IPRP) method for high‑sensitivity purity analysis of peptide–oligonucleotide conjugates (POCs). Using a model POC (R9‑ER(dec)‑R), the workflow demonstrates reliable differentiation of closely related impurities, supporting accurate quality control and improved confidence in conjugation efficiency for next‑generation oligonucleotide therapeutics.