ResourceLife Sciences
High-Fidelity Amplification of GC-Rich DNA is a Challenging Application Due to the Low Processivity and Lack of Robustness of Most Proofreading DNA Polymerases
31 Aug 2014This application note demonstrates the novel KAPA HiFi DNA Polymerase and its proprietary buffer system, which are ideally suited for the high-fidelity amplification of DNA with a GC content >65%. The unique properties of this enzyme include high processivity and increased tolerance to DNA melting agents, which enables robust, amplification of GC-rich DNA with extremely low error rates.
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DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.