High Efficiency Production of LentiviralVectors in HEK 293FT Cells Using Plasmid Co-transfection: Calcium Phosphate vs. Lipopolyplex Transfection
4 Feb 2015Lentiviral vectors are quickly becoming a vector of choice for many in vivo gene delivery and RNA interference applications. However, lentiviral vector production remains a time consuming and relatively low yield process. This study compares the efficiency of viral production using a standard calciumphosphate protocol versus a protocol using a high efficiency, low toxicity, lipopolyplex formulation (TransIT®-LT1Reagent).
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TransIT®-LT1 Transfection Reagent
Mirus Bio LLCTransIT®-LT1 Transfection Reagent from Mirus Bio- A broad spectrum, low toxicity, DNA transfection reagent • Broad Spectrum DNA Delivery - Utilize one transfection reagent and protocol for a variety of cells. • Low Cellular Toxicity - Maintain cell density and reduce experimental biases. • High Efficiency Delivery - Achieve expression in a large population of cells for experimental success. • Deliver Single or Multiple Plasmids - Suitable for many applications such as gene expression, shRNA expression, viral production, and promoter analysis.