ResourceLife Sciences
High-content screening of neuronal toxicity using iPSC-derived human neurons
15 Aug 2024The nervous system is sensitive to the toxic effects of many chemical compounds, environmental agents and certain naturally occurring substances. High-content imaging using induced pluripotent stem cells (iPSCs) of human origin can be applied to examine neurotrophic, neuroprotective, or neurotoxic effects of pharmaceutical drug candidates or environmental contaminants. Molecular Devices demonstrates neuronal toxicity screening via automated endpoint and live-cell assays using iPSCs and Molecular Devices systems and software.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High Content ImagingHigh content imaging is a method combining two or more fluorescent microscopy experiments to identify substances that alter a cell’s phenotype in a desired manner. The process is adapted to multi-well plates and both the image acquisition and analysis are automated.ScreeningUsing robotics, data processing and control software, liquid handling devices and sensitive detectors, screening allows a researcher to quickly conduct millions of chemical, genetic or pharmacological tests.