High Content Analysis of Biomarker Intensity and Distribution in 3D Microtissues Using the Operetta High Content Imaging System

In this application note InSphero and PerkinElmer present a high content screening application to analyze different characteristics of 3D microtissues with the Operetta® High Content Imaging System. Drug sensitivity of tumor microtissues was visualized and quantified by several cancer-associated biomarkers (CathepsinB activity, Hypoxia and MMP activity) using different far infrared dyes applied regularly for near infrared imaging in vivo.