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Getting consistent sequencing results from low input and degraded samples

n6 Tec., Inc
26 May 2026

This resource explores how real‑time PCR with automated normalization enables consistent sequencing performance across challenging sample types, including FFPE-derived DNA and RNA, cell‑free DNA, and single‑cell inputs. Explore how by using the icon96™ platform with AutoNorm™, workflows can dynamically adjust amplification in real time, reducing overcycling, improving reproducibility, and enabling mixed-input samples to be processed in a single run.

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icon96™

n6 Tec., Inc

Stop gambling on cycle numbers. The icon96 uses iconPCR™ technology with AutoNorm™ software to provide real-time fluorescence feedback in each of 96 independent wells, preventing under-amplification dropouts and over-amplification artifacts—delivering balanced, sequencing-ready libraries without manual quantification or normalization.

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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.GenomicsGenomics is the study of genomes, focusing on the sequencing, analysis, and interpretation of genetic material. It is key in understanding genetic diseases, evolutionary biology, and personalized medicine. Techniques like next-generation sequencing (NGS) are commonly used in genomics research. Browse our peer-reviewed product directory to find the best genomics tools, compare products, check reviews, and get pricing directly from manufacturers.PCRSample PreparationFFPEFormalin-fixed paraffin-embedded (FFPE) tissues are commonly used in medical research, particularly in histology and cancer studies. The preservation process allows long-term storage of tissue samples for later analysis. Explore FFPE research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Single Cell AnalysisSingle-cell analysis involves studying individual cells to gain insights into their behavior, gene expression, and function. This approach is valuable in cancer research, stem cell biology, and immunology. Explore single-cell analysis products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Consistent sequencing from low input and degraded samples - SelectScience resources