Functional Characterisation of Recombinant Muscarinic M3 Receptors in Assay-Ready Frozen Cells using a Novel Calcium Assay Kit on the FLIPRTETRA® Instrument

This application poster describes transitioning a recently established muscarinic M3 receptor assay from one using cells in culture, to a more simple and robust FLIPRTETRA® assay using ECACC Assay-Ready frozen cells and the new FLIPR® Calcium 5 Assay Kit. Consistent muscarinic M3 receptor pharmacology between growing and frozen cells, good Z’ factors and robust assay performance is shown.