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Fluorescent western blotting – A guide to multiplexing
20 Jan 2025Discover the advantages of fluorescence western blotting, such as accurate quantitation, stable signals, and the ability to analyze multiple protein targets simultaneously on the same blot. This application note also includes detailed tips and tricks, a reference protocol, and a troubleshooting table.
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Invitrogen™ iBright™ FL1500 Imaging System
Thermo Fisher ScientificThe Invitrogen™ iBright™ FL1500 Imaging System is the premier model of the iBright Imaging Systems family, offering maximum application support including fluorescent western blot imaging with up to 4 fluorophores at a time.
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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MultiplexingMultiplexing refers to the ability to measure multiple targets or analytes simultaneously in a single experiment. This technique is valuable for high-throughput screening, diagnostics, and complex assays, as it increases efficiency and data quality. Browse our peer-reviewed product directory to find the best multiplexing products, compare tools, check reviews, and get pricing directly from manufacturers.