Fluorescence Polarization/Anisotropy Imaging with Zeiss Microscopes

G-Factor Correction with Anisotropy Standard

4 Jan 2018

In this application note, a general method is described for the determination of the instrumental correction factor G for fluorescence polarization/anisotropy experiments utilizing an anisotropy standard.

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Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Fluorescence MicroscopyFluorescence microscopy has become an essential tool in biology, as well as in materials science. The application of many fluorochromes has made it possible to identify cells and sub-microscopic cellular components with a high degree of specificity. Using multiple fluorescence labels, different probes can simultaneously identify several target molecules.
Fluorescence Polarization/Anisotropy Imaging with Zeiss Microscopes