ResourceLife Sciences
Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
14 Mar 2017Bio-Plex assays allow detection of multiple target proteins in a small volume of sample. These assays report the level of a protein that is phosphorylated at a specific site or sites, while total target assays report the level of the protein regardless of its phosphorylation state. This note describes a study testing the feasibility of multiplexing a Bio-Plex total target assay with Bio-Plex phosphoprotein assays for different target proteins and was designed to answer two questions:
- Can a total target assay be multiplexed with phosphoprotein assays?
- Can a total target assay detect differences in protein concentration among samples?
Links
Tags
ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Fluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.Protein DetectionProtein detection techniques are essential for understanding protein expression, localization, and function. Methods such as western blotting, ELISA, and mass spectrometry are commonly used in research and diagnostics. Browse our peer-reviewed product directory to find the best tools for protein detection, compare products, check reviews, and get pricing directly from manufacturers.PhosphorylationMultiplexingMultiplexing refers to the ability to measure multiple targets or analytes simultaneously in a single experiment. This technique is valuable for high-throughput screening, diagnostics, and complex assays, as it increases efficiency and data quality. Browse our peer-reviewed product directory to find the best multiplexing products, compare tools, check reviews, and get pricing directly from manufacturers.