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FAMEs Analysis Method Revalidation from Helium to Hydrogen for GC
10 Dec 2014Many labs use helium carrier gas for their GC analysis of FAMEs, but as prices continue to rise, changing carrier supply to hydrogen supplied by a gas generator is becoming more attractive to many analysts. This article shows that it is possible to revalidate your method by keeping the linear velocity of hydrogen the same as that of helium. By adopting this approach, you can a column with the same dimensions and will only need to make slight adjustments to the GC method.
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Gas ChromatographyGas chromatography (GC) is an analytical technique used to separate and quantitate mixtures of small and volatile compounds. Gas chromatographs or GC systems include components such as GC columns, detectors, pumps and autosamplers. Choose from packed or capillary GC columns, flame ionization (FID), photoionization (PID) electron capture detectors and selective or non-selective detectors. Find the best gas chromatographs in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gas Generators and AnalyzersGas generators are used for supplying or monitoring a regular flow of a high purity gas such as nitrogen, hydrogen or zero air for applications such as gas chromatography. Gas analyzers can be used in supply chain processes and laboratory experiments with portable analyzers for monitoring CO<sub>2</sub> and O<sub>2</sub> levels as well as trace gases. Regular flow can be produced with gas control modules and flow meters. Find the best gas generators and analyzers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.HydrogenHelium