Evaluation of the SpectraSlide AP-1 for accurate counting of splenocytes and isolated nuclei

Accurate quantification of tissue-derived samples such as splenocytes and isolated nuclei is essential for a variety of downstream applications, including immunological assays, single-nucleus RNA sequencing (snRNA-seq), and ATAC-seq. Because of their small size, these samples are particularly difficult for image-based cell counters to analyze reliably, requiring both sensitivity and consistency.

This application note, Logos Biosystems evaluates the performance of the SpectraSlide^® AP-1 paired with the LUNA-FX7™, focusing on its effectiveness in analyzing tissue-derived samples including splenocytes and isolated nuclei.