Evaluating the Reproducibility of Protein Extraction from Hard Tissues

5 Oct 2017

Homogenization is often the first step in a tissue proteome study. It has been shown that the accuracy of proteome studies are largely dependent on the sample preparation method utilized. The Omni International Bead Ruptor 12 offers a fast and reproducible solution for tissue homogenization resulting in high protein recovery.

Bead Ruptor 12

OMNI International Inc.

The Bead Ruptor 12 is the most powerful and economical bead mill homogenizer available today. It is an ideal solution for a variety of applications that require grinding, lysing, and homogenization of biological samples prior to molecular extraction. Using disposable sample tubes pre-filled with a variety of lysing beads, the Bead Ruptor 12 vigorously and uniformly shakes up to 16 tubes at a time providing an efficient, consistent homogenization and high-yield. Unlike most other bead mill homogenizers, the Bead Ruptor 12 does not require a cool down period between runs. This means more samples can be processed in a smaller amount of time.   Benefits of the Bead Ruptor 12 Homogenizer include: Simultaneously homogenize up to 12 x 2mL or 0.5mL tubes and 4 x 7mL tubes Convenient front-loading tube holder position for ease of use and optimal bead/sample interactions Processing range is ideal for soft tissue samples No cool-down required between runs = non-stop processing Disposable tubes ensure no threat of cross-contamination No sample degradation due to quick processing Features and integrated lid lock   Compelling performance at a compelling price The Bead Ruptor 12 is designed with a unique  tube carriage motion to ensure that the intra-tube bead movement reduces swirling and results in the  a high bead impact force. The Bead Ruptor 12 is more powerful than  competitor bead mills that are twice the cost making it an excellent solution for any lab working with tissues or cellular material. Bead impact forces generated by commercially available bead mills.   Bead impact forces are calculated as a function of tube acceleration, tube displacement and the motor force applied to the tube. The Bead Ruptor 12 and Bead Ruptor Elite have the broadest force range and produce the highest impact forces of any bead mill on the market. This results in an increased processing efficiency on the Bead Ruptor platforms   Unique snap in tube carriage design The Bead Ruptor 12 eliminates the tedious task of screwing down finger plates  which are present other bead mills. The patent pending snap in tube carriage allows tubes to be “snapped in” and “snapped out” easily thus decreasing  workload and increasing safety. The Bead Ruptor 12 is an ideal bead mill for core laboratories or as a shared resource with its short learning curve and ease of use.   Wide range of bead kits for any sample type  Omni International offers users ultimate flexibility with a range of bead beating tubes and lysing matrices to support homogenization of any sample type.

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Homogenizers and BlendersHomogenizers and blenders are used to homogenize various types of material, including tissue, cells, plants, food, soil, biological and chemical samples for subsequent analysis. Lab equipment for sample homogenization includes handheld and benchtop homogenizers, bead mill, ultrasonic and multiple sample homogenizers, high shear homogenizers and blenders. Homogenizer bead and probe multipacks are available to avoid cross contamination of samples, as well as cooling systems for homogenizing temperature-sensitive samples. Homogenizers are often used in the first step of extraction of DNA, RNA and proteins from tissue and cell samples. Find the best homogenizers and blenders in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Tissue ProcessingTissue processing involves preparing biological tissue samples for microscopic analysis, including fixation, embedding, and sectioning. This process is essential for histopathology and diagnostic applications. Explore tissue processing products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Sample HomogenizerHomogenizingProtein BiologyProtein Quantification