Enhancing Throughput with Multiple Cell Lines Per Well with the QPatch HTX
3 Apr 2011In this application note, a series of experiments with a combination of ion channels involved in cardiac risk assessment are conducted. Parallel operation of 48 multi-hole patch clamp sites, each comprising 10 individual patch clamp holes, in a single measurements site, allows for high throughput. The ion channel currents were separated temporally or pharmacologically by proper choices of voltage protocols or ion channel inhibitors. Biophysical and pharmacological data are presented. Simultaneous recordings on multiple cell lines expressing different ion channels are analysed using the QPatch HTX.
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QPatch HT and QPatch HTX
Sophion BioscienceThe QPatch HT and QPatch HTX enable high-throughput patch clamping with 48 parallel recording sites. Automated patch clamping with the same high quality afforded by conventional patch clamping can now be applied in all phases of ion channel drug discovery, including primary screening of focused compound libraries. • Up to 7000 data points per day • Screening and compound profiling • Multi-hole and single-hole mode (QPatch HTX) • Ligand- and voltage-gated recordings • Eight pipette liquid handling with intelligent scheduler • True gigaseals • Flow channels for solution exchange • Up to 50 liquid additions • User-friendly experiment execution • Unattended operation for several hours • On-board cell preparation • Elevator units for QPlate stacking • Elevator units for compound plate stacking • Cell clone screening feature • Multiple intracellular solutions testing feature • User friendly assay setup and quick data analysis