Enhanced LC-MS/MS separation of Δ⁸ and Δ⁹-THC metabolites and other cannabinoids extracted from urine samples using a polymeric SPE column
19 Oct 2023In this application note, UCT, Inc. presents a method addressing the challenge posed by the increased use of Δ⁸-THC products. Drug testing labs face the need to separate parent THC isomers Δ⁸ and Δ⁹-THC. THC metabolites, particularly isomeric ones like Δ⁸-OH-THC and Δ⁹-OH-THC, present a challenge. Additionally, new cannabinoids such as Δ10 and Δ6a,10a-THC, and THC-O-Acetate are emerging. The note introduces an LC-MS/MS method employing UCT’s SelectraCore® PFPP column to separate 16 cannabinoids, including isomeric metabolites of Δ⁸ and Δ⁹ -THC. Solid-phase extraction from urine is detailed using UCT’s Styre Screen® HLB column.
Related Products
Request Quote for All Products
Styre Screen® Extraction Columns
UCT, Inc.Styre Screen® Extraction Columns contain an ultra clean, highly cross-linked styrene and divinylbenzene copolymer. The Styre Screen particles have an average particle size of 30 microns and a very high analyte capacity making them ideal for standard solid phase extraction applications. The increased analyte capacity means that less sorbent bed mass is needed which results in faster flow rates and less solvent use. Higher throughput and less solvent waste disposal translate into significant savings in both time and money. In addition, no conditioning steps are required for most analyte applications. Styre Screen® Extraction Columns Advantages: Copolymer allows for extraction of acids, neutrals and bases High and reproducible recoveries Clean extractions Highly cross-linked styrene/divinylbenzene polymer Reduction in sorbent mass Faster flow rates pH stable (1 to 14) Reduction in solvent use High sorbent capacity Methods for NIDA/SAMHSA 5 Drugs