ELISA 101

25 Sept 2026

This eBook provides a practical introduction to enzyme-linked immunosorbent assays (ELISAs), covering the principles, workflows and applications of one of the most widely used analytical techniques in biological research. Learn about the different ELISA formats, assay setup, data analysis and automation strategies for high-throughput workflows. Discover how ELISAs are applied across fields including food safety, protein quantification, infectious disease research and biomarker detection, while gaining insight into the instrumentation and software used to streamline assay development and analysis.

Resource details:

Resource type: Application eBook
Page count: 12
Read time: 15 mins

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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.