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Easy and Quick HPLC Analysis of Aflatoxin in Milk
Easy and Quick HPLC Analysis of Aflatoxin in Milk
2 Feb 2016Aflatoxins are naturally occurring mycotoxins that are produced by different species of fungi. In addition to the most toxic aflatoxin B1, a number of other related compounds are occurring in nature. High level exposure to aflatoxins can, for example, produce acute hepatic necrosis especially children, whilst adults have higher tolerances. This application note demonstrates an HPLC method using a YMC-Triart C18 column and fluorescent detection.
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Versatile, pH Stable (U)HPLC Columns: YMC-Triart Portfolio
YMC EuropeYMC-Triart: Robust and reproducible (U)HPLC columns with a wide range of stability towards pH (1-12), temperature and 100% aqueous eluents.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Food and Beverage AnalyzersFood and Beverage Analyzers are commonly used to provide information about the chemical composition, structure and physical properties of food materials.Food and Beverage ProcessingFood and Beverage Processing involves the preparation of food products for further analysis. Typical instrumentation includes blanchers, color sorters, cookers, grinders, and refrigeration. These are used to replicate the processes commonly used in the commercial preparation of food and beverages.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Food SafetyFood safety describes the prevention of food-borne illnesses. Contamination with a variety of pathogens can be prevented with adequate temperature control, hygiene and labeling. Food samples can be tested using GC/MS, HPLC, SPE, and qPCR to ensure their adherence to regulations.MycotoxinsToxic chemicals such as Aflatoxin and Alkaloids that are produced by fungi are known as mycotoxins. In humid conditions, fungi can proliferate and colonize on crops to levels that can be damaging to human health. MilkMilk is a dairy product that contains lactose. It is consumed by infant mammals to provide nutrients from the mother. In 2008, the Chinese milk scandal saw the unlawful adulteration of powdered milk with melamine.Food ContaminationFood contamination refers to the presence of harmful substances or pathogens in food, posing risks to human health. Contaminants can be biological, chemical, or physical, and their detection is crucial for ensuring food safety. Advanced testing methods like PCR, chromatography, and spectroscopy are used to identify contaminants in food products. Browse our peer-reviewed product directory to find the best solutions for food contamination detection, compare products, check reviews, and get pricing directly from manufacturers.Aflatoxin