Direct PCR on Hair: A New Animal-Friendly Genotyping Method

Thermo Scientific Arktik Thermal Cycler is suitable for a wide range of applications from individual reactions to high-throughput projects. It offers flexibility with three different interchangeable blocks: 96-well block, 384-well block, and a dual block with two 48-well units. A broad temperature gradient in 96 and 384 blocks simplifies temperature optimization. Ease-of-use is the key feature of the Arktik Thermal Cycler’s user interface. The protocol is displayed graphically, and the programming is simple with intuitive menus. Highlights of the Arktik Thermal Cycler: Interchangeable blocks with excellent thermal precision Dual 48-well block for multi-user option Broad (up to 30°C) and accurate temperature gradient† Reliable and easy to use Low noise level Accepts virtually all standard PCR plastics †In 96- and 384-well blocks. Gradient feature not available in the U.S., Germany, and Japan. Flexibility with interchangeable blocks Interchangeable blocks allow the Arktik Thermal Cycler to be quickly and easily transformed from a 96-well to a 384-well PCR instrument. With the dual block, the instrument operates as two 48-well units, enabling two independent protocols to be performed simultaneously. The blocks feature a patent pending heat sink design that improves the speed and thermal uniformity of the system. Broad and accurate temperature gradient speeds up protocol optimization* Tedious temperature optimization can be simplified to a single experiment with the Arktik Thermal Cycler’s advanced gradient feature. The gradient range is broad – up to 30°C – and accurate, allowing easy temperature optimization. Reliable and quiet instrument for demanding use State-of-the-art Peltier technology employed in heating and cooling of the blocks ensures that the instrument maintains its high level of performance in demanding use. An overtightening protection system in the heated lid simplifies setting the correct pressure for PCR vessels used. The low noise level of the instrument is an additional benefit when the instrument is placed in the laboratory working space.

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects. The Phire® Animal Tissue Direct PCR Kit employs Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA-binding domain that enhances the processivity of the polymerase. Phire Hot Start II DNA Polymerase also exhibits extremely high resistance to many PCR inhibitors found in animal tissues.The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized reagents, sampling tools, and DNARelease Additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes detailed instructions and control primers that can be used with numerous animal species.Phire Animal Tissue Direct PCR Kit Features & Benefits: No need for time-consuming and expensive DNA purification steps Very little sample material required Two simple protocols for various applications Convenient sampling tools included in the kit Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb). Minimized pipetting with master mix format and loading dyes pre-added Extremely short PCR protocol times Tested with wide variety of sample types Robust hot start DNA polymerases guarantee high yields of specific product Rich composition of the kit - controls, water and DNA ladder included Phire Animal Tissue Direct PCR Kit Includes: Phire Hot Start II DNA Polymerase 2X Phire Animal Tissue PCR Buffer (includes dNTPs and MgCl2) Universal control primer mix Dilution Buffer DNARelease Additive Gel loading dye Harris Uni-Core™ 0.50mm Harris Cutting Mat™