Development of Integrated Informatics Workflows for the Automated Assessment of Comparability for Antibody Drug Conjugates (ADCs) Using LC-UV and LC-UV/MS

Antibody Drug Conjugates (ADCs) have been emerging as a new class of anti-cancer therapeutics, combining the targeted delivery capability of a monoclonal antibody (mAb) with the potency of conjugated cytotoxic agents. By releasing the cytotoxic drugs inside target tumor cells, ADCs minimize exposure of non-target cells to toxic drug payloads and thereby reduce potential side effects of the treatment. This application note demonstrates an integrated informatics workflow using UNIFI® Software that streamlines data acquisition, processing, and reporting for ADC analysis by LC-UV or LC-MS, so that CQAs information, such as average DAR and drug load distribution for multiple batches of ADCs, from multiple analytical techniques can be effectively compiled for rapid quantitative assessment.