ResourceLife Sciences
Developing Assays for Screening GPCRs with Transiently Transfected Cells Using the MaxCyte® STX™ System
14 Aug 2014This application note demonstrates that the MaxCyte STX transient transfection system enables rapid development of cell-based assays to screen GPCRs and other target molecules. Cells transfected with the MaxCyte STX system are suitable for screening with a variety of automated assay technologies, such as the FLIPRTETRA®. MaxCyte STX users can exert tight control over assay sensitivity and dynamic range by varying the concentration of plasmid DNA in the electroporation reaction.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.TransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.gPCRElectroporation