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Determination of Hydroxymethylfurfural in Honey Using the LAMBDA Spectrophotometer
Determination of Hydroxymethylfurfural in Honey Using the LAMBDA Spectrophotometer
21 Dec 2015In this application note, the Hydroxymethylfurfural (HMF) content has been determined using the LAMBDA™ 465 UV/Vis Spectrophotometer and the Equation Calculation mode in the UV Lab™ software. Normally, HMF is generated by the decomposition of fructose in acid conditions. It occurs naturally in most honeys and increases rapidly with heat treatment of honey. Therefore, it can be used as an indicator of heating and storage time at elevated temperatures. Good quality honey has a lower amount of HMF; for that reason, the level of HMF present is a key indicator as to the quality of honey.
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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
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