Detection of Birch Pollen Allergen in the Air: First Feasibility Study

This application note shows that Surface Plasmon Resonance imaging (SPRi) is a suitable technique to detect allergens. Compared with other techniques, such as ELISA or Luminex, which are based on colorimetric or fluorescence detection, this technology does not require the labeling of antibodies. We focus here on the real-time detection, in a multiplex format, of a recombinant birch pollen allergen (rBet v 1) and anatural birch pollen allergen (Bet v 1) contained in pollen grains by monitoring the kinetics of the interaction between an anti-Bet v 1 antibody and the birch pollen allergens. This unique technique allows, for the first time, the combination of both real-time and multiplex detection of pollen allergens.