Culture medium analysis for a metabolic analysis of antibody-producing cells using LC-MS/MS and ICP-MS

This application note from Shimadzu describes how to use LC-MS/MS (LCMS-8060NX) to analyze 144 organic compounds and ICP-MS (ICPMS-2030) to analyze 9 inorganic elements in the culture medium and culture supernatant of a CHL-YN antibody producing cell line culture. This app note also describes how to use this data to identify which components and metabolic pathways are involved in antibody production.