Couple Any Ligand to Your Particle of Choice

20 Sept 2016

This application note, shows a range of proteins covering several species and isotypes of antibodies, as well as bacterial recombinant proteins which can be successfully coupled to Anteo activated particles with minimal optimization. It also shows that different proteins do have different affinities for Anteo coupling, and can be titrated to produce multifunctional particles.

Magnetic Separation Coupling Kit

Anteo Technologies

Reduce labor costs by using our ready-to-use Magnetic Separation Coupling Kit. No messy pre-preparation of reagents required. Simply pipette Anteo's water-based Activation Reagent onto your chosen particle to activate. Hands-on time for surface activation and coupling is 60 minutes. This kit greatly reduces the risk of antibody damage, leading to less antibodies required for the experiment, increased antibody functionality, reduced interference and increased uniformity between experiments. This Anteo kit is also essential for exploratory work. Removing R&D steps required for this type of work, this Kit enables scientists to achieve results even when testing with unknown proteins. The Anteo Magnetic Bead Separation Kit is compatible with Carboxyl beads to give scientists a cost effective option, while supporting their experiments with all the benefits that Anteo’s technology provides.   Features: Hand on time for surface activation and coupling is 60 minutes Essential for exploratory work, achieve results when testing with unknown proteins Couple any ligand to your particle of choice Allows the development of multi-functional coupled particles with easy titration of ligand coupling concentration No pre-treatment of proteins or antibody is required to achieve results An alternative to covalent NHS/EDC and passive binding the Anteo Kit is easy to use, while reducing damage to antibodies Less hazardous chemicals to work with than NHS/EDC, Anteo’s Activation Reagent is water based Easy to use: Simply pipette the Activation Reagent on your surface to activate Promotes monolayer binding which increases antibody functionality Reduces aggregation and interference Increases uniformity between experiments Supports multi-functional protein separation  

(0)

Links

Tags

AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.NanotechnologyNanotechnology, or nanotech, is an engineering technique using molecular scale functional systems. Applications of nanotechnology include medicine and medical devices, electronics, air and water purification, food science and energy production.Surface Area TestingPhysisorption studies fundamental parameters essential for the characterization of materials such as the specific surface area and pore size distribution. Properties such as porosity, strength, hardness, permeability, separation selectivity, corrosion, and thermal stress resistance can all be directly correlated to the porous structure of a material.Magnetic BeadsProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.Antibody ArraysConjugation
Couple Any Ligand to Your Particle of Choice