Correlation of Angiogenesis Biomarkers with Early Metastatic Progression in NSCLC as Determined using a Mulitplexed Immunoassay Kit

6 Jun 2016

This white paper demonstrates the general, practical ability of MILLIPLEX® MAP assay kits to generate data that can directly enhance studies of cancer metastasis.

MILLIPLEX MAP Human Complement Panel 1 - Immunology Multiplex Assay

Merck

EMD Millipore’s MILLIPLEX® MAP Complement Panel 1 Magnetic Bead Panel is an 8-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: Complement 2, Complement C4B, Complement C5, Complement C5a, Complement C9, Adipson/Complement Factor D, Mannose-Binding Lecting (MBL) and Complement Factor I. MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior(e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. Each MILLIPLEX® MAP panel and kit includes: Quality controls (QCs) provided to qualify assay performance Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency Optimized serum matrix to mimic native analyte environment Detection antibody cocktails designed to yield consistent analyte profiles within panel EMD Millipore’s MILLIPLEX® MAP Complement Panel 1 Magnetic Bead Panel is part of the most versatile system available for complement factor research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.

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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.Angiogenesismultiplex assayCancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.