Comprehensive Determination of Trans Fats in Cookies using SP-2560 and SLB-IL111 GC Columns after Silver-Ion SPE Fractionation
15 Oct 2014This application note demonstrates the extraction of lipids as fatty acids, and subsequent detection of trans fats from a cookie sample made using partially hydrogenated vegetable oils. Ag-Ion SPE was used to fractionate the extracted sample into saturated FAMEs, and trans monoenes, cis monoenes, and dienes. Subsequent analysis of the monoene fractions on a highly polar SP-2560 column and an extremely polar SLB-IL111 column provided resolution of many of the C18:1 cis/trans geometricpositional isomers, with different elution patterns observed.
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SP-2560 Capillary GC Column
Sigma-Aldrich SupelcoSP-2560 Capillary GC Column• Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs). It is extremely effective for FAME isomer applications.• USP Code: This column meets USP G5 requirements.• Phase: Non-bonded; poly(biscyanopropyl siloxane)• Temp. Limits: Subambient to 250 °C (isothermal or programmed
SLB®-IL111 Capillary GC Column
Sigma-Aldrich SupelcoSLB®-IL111 Capillary GC Column for FAME and Petroleum Applications. World’s first commercial column to rate over 100 on our GC column polarity scale. Selectivity most orthogonal to non-polar and intermediate polar phases, resulting in very unique elution patterns. Maximum temperature of 270 ºC is very impressive for such an extremely polar column. Great choice for separation of polarizable analytes (contain double and/or triple C-C bonds) from neutral analytes. Also a good GCxGC column choice.