Comprehensive Characterization of Trastuzumab using a Single-Shot Peptide Mapping Approach with CESI-MS
25 Nov 2014Capillary electrophoresis (CE) has exceptional separation efficiency and capabilities for peptide mapping of mAbs by mass spectrometry. Technology involving an electrospray ionization (ESI) emitter integrated with capillary electrophoresis (CE) has been developed, combining CE separation and ESI into a single dynamic process (CESI). This poster presents data for the bottom-up proteomic peptide mapping of a leading, representative monoclonal antibody, Trastuzumab (Herceptin) using a single protease, trypsin. A Beckman Coulter CESI 8000 system sold through SCIEX Separations, a part of AB SCIEX, coupled to a Thermo Q-Exactive was used for the analysis.
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CESI 8000 Plus system
SCIEXExtending the reach of your mass spectrometer. Mass spectrometry (MS) has become an indispensable technology for the analysis of compounds of biological interest. Our improvements in assay sensitivity and reduction in ion suppression reveal new information that is critical to your research, whether you’re characterizing a therapeutic protein, identifying the proteins that make up a specific proteome, analyzing post-transitional modifications, or studying a metabolomic fingerprint. We developed a new front-end separation and ionization technology called CESI, which combines the high efficiency and ultra-low flow of capillary electrophoresis (CE) with an integrated electrospray ionization source. This optimization of electrospray ionization (ESI) into the nanoflow region has been shown to greatly improve assay sensitivity and reduce ion suppression.