ResourceLife Sciences
Competitive Analysis of a Small Molecule Model System
23 Sept 2012Surface Plasmon Resonance (SPR) is a well established technique for monitoring biomolecular interactions. However, the sensitivity of SPR is often limited when working with small molecules. In this application note, the analysis of small molecules with SPR using a competitive assay format and Episentec dyes is described. It is shown that competitive analysis with small dye labels enables both equilibrium and kinetics to be studied.
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BiosensorsBiosensors are devices used to detect an analyte using biological molecules specific to the analyte coupled to a detector. Biosensor instruments may be photometric, typically using surface plasma resonance (SPR), electrochemical or QCM (quartz crystal microbalance) biosensors. Biosensors should be selective, portable, robust and sensitive and have a fast response time. Systems may be manual or automated and usually have associated software.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Surface Plasmon Resonance