SelectScience
Join Free
ResourceLife Sciences

Comparison of the Oris™ Cell Migration Assay to the Scratch Assay

AMSBIO
22 Oct 2014

Cell migration is integral to many physiological processes, including embryonic development, tissue regeneration, and wound healing. In addition, cell migration is involved in tumor metastasis and atherosclerosis. One assay commonly used to study cell migration in vitro is the scratch assay which has a number of limitations. Methods for creating the scratch vary from lab to lab and results can be highly variable. Furthermore, the process of scratch formation has been shown to damage the underlying extracellular matrix (ECM). The Oris™ Cell Migration Assay (CMA) was designed to address the limitations of the scratch assay. Assay reproducibility is greater in the Oris™ CMA compared to the scratch assay as a result of uniformly sized Detection Zones, and the underlying ECM is not damaged by the silicone stopper. This application note offers a direct comparison of the Oris™ CMA and the scratch assay in assessing cell migration. Furthermore, ECM integrity is assessed in both assay formats.

Links

AMSBIOCompany website

Tags

Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell MigrationAssay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.

Related resources

Comparison of the Oris™ Cell Migration Assay to the Scratch Assay