Comparison of ionization types and precursor ions for quantitative analysis of 25-hydroxyvitamin D on a TSQ Altis MD mass spectrometer

In this application note, Thermo Fisher Scientific compares variations of an analytical method for detecting and quantifying 25-hydroxyvitamin D₂ and D₃ using a Thermo Scientific™ TSQ Altis™ MD mass spectrometer. The note emphasizes the essential role of vitamin D in various biological functions, including promoting the absorption of calcium and supporting healthy bone growth. It highlights that vitamin D deficiency can lead to weakened bones, with manifestations like rickets in children and osteomalacia in adults, causing soft and malformed bones, reduced growth, and muscle weakness.