Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
29 Mar 2017A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy. In this application note, the SNAP i.d.® 2.0 Protein Detection System for multi-slide screening with immunohistochemistry (IHC) is utilized to perform screening of an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples, so as to determine their relevance to disease.
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SNAP i.d. 2.0 IHC System
MerckIssues with Your Tissues? Process all Your Slides in a SNAP for Streamlined IHC. The SNAP i.d.® 2.0 Protein Detection System for Immunohistochemistry (IHC) introduces a new capability to the innovative, vacuum-driven SNAP i.d.® 2.0 system. The IHC slide holders allow you to block, probe, and stain up to 12 tissue slides per side (24 slides if both sides are used). Reduced handling time and multiple-slide processing make this system ideal for when you are optimizing antibody conditions and protocols.With two individually controlled sides, the system base allows for independent, vacuum-driven processing of either one or two IHC frames. Each of the IHC frames can process between 1 to 12 glass slides through independent vacuum ports.Each slide holder has an injection/recovery port that enables the manual addition, as well as the removal and recovery, of small volumes of antibodies or reagents; reagents can also be flushed using the vacuum feature if conservation is not a priority.Benefits: Eliminates the need for pap pens Antibodies can be collected and reused Slide handling time is significantly decreased Less time spent on wash steps Parallel processing of multiple slides Features: Flexibility of multiple slide configurations enables the processing of 1 to 24 slides at a time Compatible with standard IHC slides and protocols Compatible with diverse tissue preparations including formalin-fixed or fresh frozen samples Intuitive format Incorporates blocking, washing, and antibody incubation and labelling steps Systematizes handling multiple slides without the cost of automation Test tracker feature on frame cover helps keep track of IHC steps